Methodological hallmarks

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Human

Underestimation of hepcidin concentration by time of flight mass spectrometry and competitive ELISA in hepcidin p.Gly71Asp heterozygotesvan der Vorm LN, van Meijel LJ, Lips J, Galesloot TE, Laarakkers CM, Swinkels DW. Clin Chem Lab Med 2015.
Engineered human lipocalin as an antibody mimetic: application to analysis of the small peptide hormone hepcidinGrebenchtchikov N, Geurts-Moespot AJ, Trentmann S, Andersen N, Bel Aiba RS, Allersdorfer A, Laarakkers CM, Sweep FC, Tjalsma H, Hohlbaum AM, Swinkels DW. Clin Chem 2014;60(6):897-9.
Improved mass spectrometry assay for plasma hepcidin: detection and characterization of a novel hepcidin isoformLaarakkers CM, Wiegerinck ET, Klaver S, Kolodziejczyk M, Gille H, Hohlbaum AM, Tjalsma H, Swinkels DW. PLoS One 2013;8(10):e75518.
Immunochemical and mass-spectrometry-based serum hepcidin assays for iron metabolism disordersKroot JJ, Laarakkers CM, Geurts-Moespot AJ, Grebenchtchikov N, Pickkers P, van Ede AE, Peters HP, van Dongen-Lases E, Wetzels JF, Sweep FC, Tjalsma H, Swinkels DW. Clin Chem 2010;56(10):1570-9.
Immunochemical and mass-spectrometry-based serum hepcidin assays for iron metabolism disordersGrebenchtchikov N, Geurts-Moespot AJ, Kroot JJ, den Heijer M, Tjalsma H, Swinkels DW, Sweep FG. Br J Haematol 2009;146(3):317-25.
Advances in quantitative hepcidin measurements by time-of-flight mass spectrometrySwinkels DW, Girelli D, Laarakkers C, Kroot J, Campostrini N, Kemna EH, Tjalsma H. PLoS One 2008;3(7):e2706.
Novel urine hepcidin assay by mass spectrometryKemna E, Tjalsma H, Laarakkers C, Nemeth E, Willems H, Swinkels D. Blood 2005;106(9):3268-70.

Standardization

Diurnal rhythm rather than dietary iron mediates daily hepcidin variationsSchaap CC, Hendriks JC, Kortman GA, Klaver SM, Kroot JJ, Laarakkers CM, Wiegerinck ET, Tjalsma H, Janssen MC, Swinkels DW. Clin Chem 2013;59(3):527-35.
Improved mass spectrometry assay for plasma hepcidin: detection and characterization of a novel hepcidin isoformLaarakkers CM, Wiegerinck ET, Klaver S, Kolodziejczyk M, Gille H, Hohlbaum AM, Tjalsma H, Swinkels DW. PLoS One 2013;8(10):e75518.
(Pre)analytical imprecision, between-subject variability, and daily variations in serum and urine hepcidin: implications for clinical studiesKroot JJ, Hendriks JC, Laarakkers CM, Klaver SM, Kemna EH, Tjalsma H, Swinkels DW. Anal Biochem 2009;389(2):124-9.

Reference values

Serum hepcidin measured by immunochemical and mass-spectrometric methods and their correlation with iron status indicators in healthy children aged 0.5-3 yUijterschout L, Swinkels DW, Domellöf M, Lagerqvist C, Hudig C, Tjalsma H, Vos R, van Goudoever JB, Brus F. Pediatr Res 2014;76(4):409-14.
The iron regulatory hormone hepcidin is decreased in pregnancy: a prospective longitudinal studyvan Santen S, Kroot JJ, Zijderveld G, Wiegerinck ET, Spaanderman ME, Swinkels DW. Clin Chem Lab Med 2013;51(7):1395-401.
Iron status and systemic inflammation, but not gut inflammation, strongly predict gender-specific concentrations of serum hepcidin in infants in rural KenyaJaeggi T, Moretti D, Kvalsvig J, Holding PA, Tjalsma H, Kortman GA, Joosten I, Mwangi A, Zimmermann MB. PLoS One 2013;8(2):e57513.
Serum hepcidin: reference ranges and biochemical correlates in the general populationGalesloot TE, Vermeulen SH, Geurts-Moespot AJ, Klaver SM, Kroot JJ, van Tienoven D, Wetzels JF, Kiemeney LA, Sweep FC, den Heijer M, Swinkels DW. Blood 2011;117(25):e218-25.

Round robins

Toward Worldwide Hepcidin Assay Harmonization: Identification of a Commutable Secondary Reference Material.van der Vorm, Hendriks, Laarakkers, Klaver, Armitage, Bamberg, Geurts-Moespot, Girelli, Herkert, Itkonen, Konrad, Tomosugi, Westerman, Bansal, Campostrini, Drakesmith, Fillet, Olbina, Pasricha, Pitts, Sloan, Tagliaro, Weykamp, Swinkels. Clin Chem 2016;62
Second round robin for plasma hepcidin methods: first steps toward harmonizationKroot JJ, van Herwaarden AE, Tjalsma H, Jansen RT, Hendriks JC, Swinkels DW. Am J Hematol 2012;87(10):977-83.
Results of the first international round robin for the quantification of urinary and plasma hepcidin assays: need for standardizationKroot JJ, Kemna EH, Bansal SS, Busbridge M, Campostrini N, Girelli D, Hider RC, Koliaraki V, Mamalaki A, Olbina G, Tomosugi N, Tselepis C, Ward DG, Ganz T, Hendriks JC, Swinkels DW. Haematologica 2009;94(12):1748-52.

Animal

The effects of the anti-hepcidin Spiegelmer NOX-H94 on inflammation-induced anemia in cynomolgus monkeysSchwoebel F, van Eijk LT, Zboralski D, Sell S, Buchner K, Maasch C, Purschke WG, Humphrey M, Zöllner S, Eulberg D, Morich F, Pickkers P, Klussmann S. Blood 2013;121(12):2311-5.
Iron supplementation in suckling piglets: how to correct iron deficiency anemia without affecting plasma hepcidin levelsStarzyński RR, Laarakkers CM, Tjalsma H, Swinkels DW, Pieszka M, Styś A, Mickiewicz M, Lipiński P. PLoS One 2013;8(5):e64022.
Mass spectrometry analysis of hepcidin peptides in experimental mouse modelsTjalsma H, Laarakkers CM, van Swelm RP, Theurl M, Theurl I, Kemna EH, van der Burgt YE, Venselaar H, Dutilh BE, Russel FG, Weiss G, Masereeuw R, Fleming RE, Swinkels DW. PLoS One 2011;6(3):e16762.

Reviews

Hepcidin in human iron disorders: diagnostic implicationsKroot JJ, Tjalsma H, Fleming RE, Swinkels DW. Clin Chem 2011;57(12):1650-69
Hepcidin: from discovery to differential diagnosisKemna EH, Tjalsma H, Willems HL, Swinkels DW. Haematologica 2008;93(1):90-7.

Pathophysiology

Secretion of bioactive hepcidin-25 by liver cells correlates with its gene transcription and points towards synergism between iron and inflammation signaling pathwaysKartikasari AE, Roelofs R, Schaeps RM, Kemna EH, Peters WH, Swinkels DW, Tjalsma H. Biochim Biophys Acta 2008;1784(12):2029-37.

Inflammatory and infectious disorders

Secretion of bioactive hepcidin-25 by liver cells correlates with its gene transcription and points towards synergism between iron and inflammation signaling pathwaysKartikasari AE, Roelofs R, Schaeps RM, Kemna EH, Peters WH, Swinkels DW, Tjalsma H. Biochim Biophys Acta 2008;1784(12):2029-37.